Tempus Publications

medRxiv, Tempus-authored — We performed a retrospective analysis of longitudinal real-world data (RWD) from breast cancer patients to replicate results from clinical studies and demonstrate the feasibility of generating real-world evidence. We also assessed the value of transcriptome profiling as a complementary tool for determining molecular subtypes. PATIENTS AND...

Journal of Cutaneous Pathology, Tempus-authored — Deep penetrating nevi (DPN) are dermal‐based, heavily pigmented melanocytic proliferations primarily resulting from mutations in B‐catenin and BRAF or, less commonly, NRAS. DPNs are considered to be intermediate grade tumors which are stable with low risk of malignant tra...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Background: We recently reported two ESR1 fusions (ESR1-YAP1 and ESR1-PCDH11X) that drive endocrine therapy (ET) resistance and metastasis in estrogen receptor positive (ER+) metastatic breast cancer (MBC) (PMC6171747). Here we report additional ESR1 fusions wit...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Early detection of recurrence is critical to improve the survival of cancer patients. Blood-based “liquid biopsies” provide a minimally invasive method to detect variants, and ultra low-pass whole-genome sequencing (LPWGS) can accurately and sensitively detect low fractions of circulating tumor...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Background: Patient derived tumor organoids (TOs) are emerging as potential models to elucidate mechanisms of tumor biology and therapeutic response. Here, we establish pan-cancer metrics for validation of genetic and transcriptomic recapitulation, and concordance of an organoid to its native tumor...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Background: Tumors of unknown origin account for up to 5% of newly diagnosed cancers and the average survival time is 9 to 12 months from diagnosis. Establishing tumor type and subtype guides standard of care treatment for several NCCN targeted therapy guidelines. Metho...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Gene fusions can serve as key drivers in the development of various cancers and represent important therapeutic targets and diagnostic biomarkers. Due to high detection of candidate fusions from RNA-sequencing data, there is a recognized need to build tools that...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Patient derived Tumor Organoids (TOs) are emerging as patient-representative models that recapitulate clinical responses to candidate therapeutics. Yet standard methods of interpreting in vitro treatments of TOs have been developed from monoclonal, rapidly proliferating 2D cell lines that are not...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Laboratories conducting high volumes of RNA sequencing must be extremely wary of technical batch effects if samples are to be compared across extended time periods, which is imperative for the most well-powered analyses of cancer transcriptomes. Changes in reagents, protocols,...

American Association for Cancer Research Annual Meeting 2020, Tempus-authored — Transcriptome profiling of tumors by standard RNA-seq measures the average effect of the tumor microenvironment. This expression profile is largely shaped by the tumor architecture where tumor purity can directly influence the genomic interpretation and consequent associations with clinical outcomes....